Software-based three dimensional reconstructions and enhancements of focal depth in microphotographic images

Software-based, the focal depth in light microscopic photographic images can be enhanced fundamentally. Thus, three dimensional images are achievable, comparable with scanning electron microscopic results. Moreover several parameters which determine the quality of microscopic images can be improved by averaging. For these purposes, a vertical stack has to be taken as sequence or scan, consisting of several single images with different planes of focus. The single images are software-based superimposed; only regions, which are in focus, contribute to the resulting reconstructed image. Resulting images are without any unsharpness, with independence from the vertical dimension of the specimen or the magnification and focal depth of the objective.